Mannitol Salt Agar MSA is used as a selective and differential medium for the isolation and identification of Staphylococcus aureus from clinical and non-clinical specimens. It encourages the growth of a group of certain bacteria while inhibiting the growth of others. It is a selective medium prepared according to the recommendations of Chapman for the isolation of presumptive pathogenic staphylococci.
Mannitol Salt Agar contains peptones and beef extract, which supply nitrogen, vitamins, minerals and amino acids essential for growth. The 7. Sodium chloride also supplies essential electrolytes for transport and osmotic balance. Mannitol is the fermentable carbohydrate, fermentation of which leads to acid production, detected by phenol red indicator, aids in the differentiation of staphylococcal species.
Coagulase positive staphylococci e. Agar is the solidifying agent. The salt clears the egg yolk emulsion and lipase production is detected as yellow opaque zone around the colonies. Hallo, My respected sir, You please tell me… How process can I measurements the antimicrobial sensitivity test of Staphylococcus aureus??? Which process or method can I following for the sensitivity test of Staphylococcus aureus??????
I kindly requested to you, please you response me. With my respect. If the organism in MSA ferments mannitol and forms yellow colonies, can we directly assume it as S. What is the ideal method? Typically, human feces contains a large variety of Salmonella growing as black colonies on Hektoen agar.
Share This. Author: Rodney E. Rohde, Ph. Get published in an ASM journal Submit now. This medium only grows salt-loving bacteria so it is selective. In addition, bacteria that grow on MSA that can ferment mannitol, a sugar alcohol, will turn the medium from its original pink color to a bright, neon yellow. Sources and Resources. Bauman, R. Colony directly under word "MacConkey's" os a colorless non-lactose fermenter. Click here for more MacConkey's Agar Images.
Same plate from bottom.
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